Paper Information

Journal:   AVICENNA JOURNAL OF PHYTOMEDICINE (AJP)   2017 , Volume 7 , Number 1; Page(s) 73 To 79.
 
Paper:  ANTINOCICEPTIVE EFFECT OF EXTRACTS OF MARRUBIUM ASTRACANICUM JACQ. AERIAL PARTS
 
Author(s):  KAHKESHANI NILOOFAR, GHAREDAGHI MILAD, HADJIAKHOONDI ABBAS, SHARIFZADEH MOHAMMAD, KHANAVI MAHNAZ*
 
* DEPARTMENT OF PHARMACOGNOSY, FACULTY OF PHARMACY AND PERSIAN MEDICINE AND PHARMACY RESEARCH CENTER, TEHRAN UNIVERSITY OF MEDICAL SCIENCES, TEHRAN, IRAN
 
Abstract: 

Objective: The genus Marrubium is used for treatment of joint pain, gout, stomach-ache and colic in Iranian Traditional Medicine. Marrubium astracanicum Jacq. (M. astracanicum) is a native species in the flora of Iran. The aim of this study was to evaluate the antinociceptive properties of various extracts of aerial parts of M. astracanicum.
Materials and Methods: Antinociceptive activities of total hydroalcoholic extract (THE) and its n-hexane (non-polar) and residual partition (polar) fractions were analyzed using formalin test in mice. Morphine (5 mg/kg) and normal saline were used as positive and negative controls, respectively.
Results: Intraperitoneal administration of THE (50, 100 and 200 mg/kg), non-polar fraction (200 mg/kg) and polar fraction (100 and 200 mg/kg), 30 min before formalin injection, caused significant analgesic activity in acute phase (0-5 min after formalin injection) of formalin test (p<0.05 as compared to control and p>0.05 in comparison with morphine). In chronic phase (15–60 min after formalin injection), non-polar and polar fractions (50, 100 and 200 mg/kg) showed significant analgesic activity (p<0.001 as compared to control and p>0.05 in comparison with morphine).
Conclusion: Different extracts of M. astracanicum demonstrated antinociceptive activity that support the traditional usage of Marrubium genus for the treatment of arthritis, gout and other inflammatory diseases.

 
Keyword(s): MARRUBIUM ASTRACANICUM, PAIN MEASUREMENT, ANALGESIC