Paper Information

Journal:   JOURNAL OF MICROBIAL BIOTECHNOLOGY   FALL 2011 , Volume 3 , Number 10; Page(s) 23 To 26.
 
Paper:  EXTRACELLULAR PRODUCTION OF SILVER NANO-PARTICLES BY FUSARIUM OXUSPORUM LABORATORY SCALE
 
Author(s):  GHOLAMI SHABANI M.H., IMANI A., RAZZAGHI ABYANEH M., RIAZI GH., CHIANI M., KHADEMI MAZDEH S., CHAMANI M., AKBARZADEH AZIM*
 
* PILOT NANOBIOTECHNOLOGY DEPARTMENT, PASTEUR INSTITUTE OF IRAN, TEHRAN 13164, IRAN
 
Abstract: 

Background and Objective: Passing through the micro-particles to nano-particles domain, encountered to some physical changes, such as, increasing the surface area to volume ratio and appearing of quantum effects. Increasing the surface area to volume ratio results in gradual decrease of particle size and overcoming the surface atoms to inner atoms behavior. The most particular purpose of nanotechnology is the improvement of methods for producing nanostructures without using of toxic material, with less expense and wastage with controlling of size. Biologists knowing of producing of minerals in nanoscales by organisms are looking for biologic systems for synthesizing of inorganic nanoparticles. The purpose of this study, was synthesizing of Silver nanoparticles by fungus Fusarium oxusporum IRAN 81C with extreme size of 50 nm.

Materials and methods: After optimizing of growth condition in culture MGYP media contains Glucose 10 g/l, peptone 5 g/l, yeast extract 3 g/l and malt extract 3 g/l, biomass of Fusarium oxusporum was prepared. After synthesizing of nanoparticles in solution of silver nitrate, FTIR, UV-vis spectrophotometer and TEM was done.

Results: The results of this analyses showed that in concentration of 10 M, biomas of Fusarium oxusporum IRAN 81C could synthesis of Silver nanoparticles extracellularly. As well as, it's clear that after filtration of fungal cell mass, the supernatant has ability to synthesis of silver nano-particles because the production of some proteins and enzymes in the culture medium.

Conclusion: Silver nanoparticles with extraordinary physical and chemical properties of Silver nanoparticles with size of 50 nm by Fusarium oxusporumm, studding on its functional production in industrial scale is suggested.

 
Keyword(s): EXTRACELLULAR, LABORATORY SCALE, FUSARIUM OXUSPORUM, SILVER NANOPARTICLES